Primer

Part:BBa_K4636036

Designed by: Pei-Yun, Hsieh   Group: iGEM23_NTHU-Taiwan   (2023-10-11)


PCRforAELA_fw

The sequence is complementary to the pUC19 plasmid[1] sequence and can be used to replicate the multiple cloning site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design

We used Primer 3[2] to search the suitable site and check it by IDT OligoAnalyzer™ Tool.[3]
General considerations for PCR Primers.[4]

(1) : Primer size between 25~30 bps.

(2) : Value of GC content should be 40%~60%.

(3) : 3’ end with highly GC content.

(4) : Tm value between 50~60°C.


Experiment

We failed in this step.


Reference

[1] https://www.neb.com/en/products/n3041-puc19-vector#Product Information
[2] https://primer3.ut.ee/
[3] https://sg.idtdna.com/pages/tools/oligoanalyzer
[4] https://www.addgene.org/protocols/primer-design/

[edit]
Categories
Parameters
None